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Image Search Results
Journal: The American Journal of Tropical Medicine and Hygiene
Article Title: Immunogenicity and Safety of a Tetravalent Recombinant Subunit Dengue Vaccine in Adults Previously Vaccinated with a Live Attenuated Tetravalent Dengue Vaccine: Results of a Phase-I Randomized Clinical Trial
doi: 10.4269/ajtmh.20-0042
Figure Lengend Snippet: Longitudinal immune responses as measured by PRNT50. The geometric mean titer (GMT) of neutralizing antibody of each group is shown at days 1, 15, 28, and 180 for DENV1 (A), DENV2 (B), DENV3 (C), DENV4 (D) as measured with the plaque reduction neutralization test (PRNT50). Error bars represent the 95% CI for each group. This figure appears in color at www.ajtmh.org.
Article Snippet: The samples were evaluated for DENV1-, DENV2-, DENV3-, and DENV4-neutralizing antibody by the plaque reduction neutralization test using a 50%
Techniques: Plaque Reduction Neutralization Test
Journal: The American Journal of Tropical Medicine and Hygiene
Article Title: Immunogenicity and Safety of a Tetravalent Recombinant Subunit Dengue Vaccine in Adults Previously Vaccinated with a Live Attenuated Tetravalent Dengue Vaccine: Results of a Phase-I Randomized Clinical Trial
doi: 10.4269/ajtmh.20-0042
Figure Lengend Snippet: Longitudinal plaque reduction neutralization test (PRNT50) seropositivity frequency. The frequency of seropositivity for each group at days 1, 15, 28, and 180 is shown for DENV1 (A), DENV2 (B), DENV3 (C), and DENV 4 (D). Seropositivity was defined as a PRNT50 ≥ 10. Error bars represent the 95% CI for each group.
Article Snippet: The samples were evaluated for DENV1-, DENV2-, DENV3-, and DENV4-neutralizing antibody by the plaque reduction neutralization test using a 50%
Techniques: Plaque Reduction Neutralization Test
Journal: Current Gene Therapy
Article Title: A Hantavirus Pulmonary Syndrome (HPS) DNA Vaccine Delivered Using a Spring-powered Jet Injector Elicits a Potent Neutralizing Antibody Response in Rabbits and Nonhuman Primates
doi: 10.2174/1566523214666140522122633
Figure Lengend Snippet: Neutralizing antibodies in rabbits after vaccination with SNV DNA vaccine delivered by PharmaJet IM or ID device. A ) Experimental design. Groups of four rabbits were vaccinated with 0.4 mg of pWRG/SN-M(opt) plasmid DNA using the indicated PharmaJet device. For IM, a single injection of 0.5 mL of vaccine was delivered per vaccination. For ID, a single injection of 0.1 mL was delivered per vaccination. Sera from the IM-vaccinated rabbits were collected on weeks 0, 4, 6, 8, and 10 (light grey arrows) and sera for the ID-vaccinated rabbits were collected on weeks 0, 3, 7, and 12 (dark grey arrows). B ) Neutralizing antibodies were measured by PRNT. PRNT 50 titers are shown. C ) Neutralizing antibodies were also measured by PsVNA. PsVNA 80 titers are plotted.
Article Snippet: Specifically, the
Techniques: Plasmid Preparation, Injection
Journal: Current Gene Therapy
Article Title: A Hantavirus Pulmonary Syndrome (HPS) DNA Vaccine Delivered Using a Spring-powered Jet Injector Elicits a Potent Neutralizing Antibody Response in Rabbits and Nonhuman Primates
doi: 10.2174/1566523214666140522122633
Figure Lengend Snippet: Hantavirus DNA vaccine delivered to NHP using PharmaJet IM v1.0 or ID v1.0 needle-free jet injection device. A. Groups of three NHP were vaccinated with the SNV DNA vaccine (pWRG/SN-M[opt]) using either the PharmaJet IM v1.0 or ID v1.0 device three times at 4-week intervals (arrows). Each vaccination consisted of two administrations of 0.5 mg of DNA/administration, one to each triceps muscle. B . SNV PsVNA 80 titers were determined and plotted. The animal ID numbers and device (IM or ID) are shown in legend. C. SNV PRNT 50 titers were determined and plotted to quantify the capacity of the sera to neutralize authentic virus.
Article Snippet: Specifically, the
Techniques: Injection
Journal: Current Gene Therapy
Article Title: A Hantavirus Pulmonary Syndrome (HPS) DNA Vaccine Delivered Using a Spring-powered Jet Injector Elicits a Potent Neutralizing Antibody Response in Rabbits and Nonhuman Primates
doi: 10.2174/1566523214666140522122633
Figure Lengend Snippet: Delivering the HPS DNA vaccine as a mixture, or to separate sites. A ) Experimental design. Rabbits were vaccinated on week 0, 4, and 8 with: Group 1: HPS vaccine (1:1 mixture of SNV DNA vaccine, pWRG/SN-M(opt); and ANDV DNA vaccine, pWRG/AND-M(opt2)) delivered to two sites; Group 2: the ANDV DNA vaccine alone (1 site); Group 3: the SNV DNA vaccine alone (1 site); or Group 4: the ANDV and SNV DNA vaccine to two separate sites. Sera were collected on indicated weeks (grey arrows). In all groups, 2 mg of DNA was injected in a 0.5 mL volume per administration. Groups 1 and 4 received 2 administrations per vaccination, one to the right and left lateral thigh muscle. Groups 2 and 3 received a single administration per vaccination. B ) PsVNA 80 titers for SNV and ANDV are shown for weeks 4, 8, and 12. Geometric mean titers +/- 95% confidence intervals are shown. Significant differences between delivery devices are shown p <0.05 *, as determined by Wilcoxon Test. Titers above 1,000 (large-dashed line) are considered high-titer. Admin= administrations, vacc= vaccination, wk= week.
Article Snippet: Specifically, the
Techniques: Injection